Journal: PLoS ONE

Article Title: Innate Immune Responses after Airway Epithelial Stimulation with Mycobacterium bovis Bacille-Calmette Guérin

doi: 10.1371/journal.pone.0164431

Figure Lengend Snippet: (A) CXCL8 and (B) IL-6 production from epithelial cells stimulated with BCG in combination with IL-17A and/or IFN-γ for 0, 24, 48 and 72 hours, MOI 1:1. The 72 hours time points are in addition represented as bar graphs for (C) CXCL8 and (D) IL-6. The results are depicted as mean ± SD of three experiments. Means were compared with one way ANOVA followed by multiple comparisons test with Tukey’s correction and significance was accepted at *p < 0.05, **p< 0.01, or ***p < 0.001.

Article Snippet: For the cytokine stimulation experiments, recombinant IL-17A and/or IFNγ (10 ng/mL, 7955-IL, 285-IF, RnD Systems, Denmark) were added to the bottom insert 30 min before the addition of BCG.

Techniques:

Journal: PLoS ONE

Article Title: Innate Immune Responses after Airway Epithelial Stimulation with Mycobacterium bovis Bacille-Calmette Guérin

doi: 10.1371/journal.pone.0164431

Figure Lengend Snippet: Primary neutrophils were stimulated with BCG for one, two and three hours in the presence of IL-17A and/or IFN-γ. (A) NETs were studied in a light microscope after 2 hours and stained with DAPI and (B) large NET structures were observed in addition to single NETs. (C) Quantification of NETs was performed with Picogreen assay on cell supernatants after 3 hours and (D) over time at 1, 2 and 3 hours. Results are depicted as mean ± SD from a total of 4 donors. Means of samples were were compared with negative control using ANOVA followed by Dunnet’s multiple comparisons test and significance was accepted at *p < 0.05, **p< 0.01, or ***p < 0.001. Bars represent 25 μm (A, B left panel) or 100 μm (B, right panel)

Article Snippet: For the cytokine stimulation experiments, recombinant IL-17A and/or IFNγ (10 ng/mL, 7955-IL, 285-IF, RnD Systems, Denmark) were added to the bottom insert 30 min before the addition of BCG.

Techniques: Light Microscopy, Staining, Picogreen Assay, Negative Control

Journal: Journal of Inflammation Research

Article Title: Generation and characterization of ixekizumab, a humanized monoclonal antibody that neutralizes interleukin-17A

doi: 10.2147/JIR.S100940

Figure Lengend Snippet: In vitro binding parameters of ixekizumab to human, cynomolgus monkey, and rabbit IL-17A determined using surface plasmon resonance

Article Snippet: Recombinant mouse IL-17A (R&D Systems) was used as a positive control for these experiments.

Techniques: In Vitro, Binding Assay